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. 2014 Sep 9;14:334. doi: 10.1186/1472-6882-14-334

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© Engel et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Monitoring of adhesion related proteins. A: Cell membrane associated (cell surface) β1-integrin expressions of plant extract treated (10 μg/ml) MCF-7 cells in percent of control measured by flow cytometry. As control DMSO treated cells were used. (mean ± SD, n = 3, **P < 0.01, *P < 0.05, significantly different compared to control, unpaired t-test). B: Fluorescence microscopy of F-actin (green) alterations after treatment with 10 μg/ml of the plant extract. Nuclei are marked in blue. Arrows point to the increased actin accumulation at sides of cell-cell contacts.