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. 2014 Sep 9;14:334. doi: 10.1186/1472-6882-14-334

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© Engel et al.; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Determination of IC ₅₀ values. The 50% inhibition concentration (IC₅₀) values of the four plant extracts JCP1, PS, ZI, JCP2 on MCF-7 cancer were measured by MTS assay. The four extracts reduced viability of MCF-7 cells in a dose dependent manner. IC₅₀ values (see Table 1) were calculated from the dose response curves by nonlinear regression. Background absorbance was corrected against MTS reaction without cells and control treatment (0.1% DMSO).