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. 2013 Nov 14;4:3. doi: 10.1186/2042-4280-4-3

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Copyright © 2013 Yaiw et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunoreactivity and grading of human cytomegalovirus (HCMV) antigens and inflammatory markers in human carotid atherosclerotic plaques as assayed by immunohistochemistry (IHC) staining. (A) Summary of IHC results on viral immediate early (IE), late (LA), 5-LO, CD3 and CD68 in plaques. (B-E) Panel of controls consist of omitting primary antibody (or Tris-buffered saline, TBS only) instead of IE (B), isotype control for LA (C), isotype control for IE (D), and virus-infected endothelial cells (E). (F-G) Immunoreactivity and grading of IE (F) or LA (G) in plaques. (H) Association between HCMV IgG antibodies and its IE antigens burden. Positivity was revealed by diaminobenzidine (DAB), brown products; Optical density, O.D.