Skip to main content
. 2013 Dec 2;7:29. doi: 10.1186/1754-1611-7-29

Figure 2.

Figure 2

Nucleotide sequences of the Bacillus BioBrick Box promoter collection. Sequences are aligned according to their -35 and -10 region. All promoter fragments were designed with 3′-end exactly upstream of the RBS. The -10 and -35 regions (if conserved) are shown in bold and underlined. Regulator binding sites for XylR in PxylA and LiaR in PliaI are shown in italics and underlined. The native PxylA also contains a catabolite responsive element (CRE) that suppresses the transcription in the presence of glucose [53]. This binding site is downstream of the promoter and is not present in the PxylA –fragment used here, which is derived from the pXT vector [39].