Figure 4.
Luciferase activity of the bacitracin-inducible PliaI promoter. Measurement of PliaI activity in pBS3Clux upon induction with various bacitracin concentrations indicated in the legend. Addition of water was used as non-induced reference. For details of growth and measurement conditions, see legend of Figure 3 and Methods. (A)-(C) Growth in LB medium. (D)-(F) Growth in MCSE medium. (A) and (D) Growth curves (OD600) from cultures of the reporter strain TMB1858 (PliaI-lux). (B) and (E) Promoter activities upon bacitracin induction expressed as luminescence in RLU/OD600.(C) and (F) Dose–response curves one hour post-induction in RPUs (normalized to PliaG). The data on induction with 100 μg/ml bacitracin was omitted in (B) and (E) due to lysis of the culture. All graphs show mean values and standard deviations (error bars) of four biological replicates.