Table 1.
Overview of the Bacillus BioBrick Box including a summary of the features
| BioBrick | Description and comments a | Reference of source | BGSC # | Registry # |
|---|---|---|---|---|
|
Vectors | ||||
| pBS1C |
Empty vector, integration at amyE, ampr, cmr |
pDG1662-derivative [23] |
ECE257 |
BBa_K823023 |
| pBS2E |
Empty vector, integration at lacA, ampr, mlsr |
pAX01-derivative [24] |
ECE258 |
BBa_K823027 |
| pBS4S |
Empty vector, integration at thrC, ampr, specr |
pDG1731-derivative [23] |
ECE259 |
BBa_K823022 |
| pBS1ClacZ |
lacZ-reporter vector, integration at amyE, ampr, cmr |
pAC6-derivative [25] |
ECE260 |
BBa_K823021 |
| pBS3Clux |
lux-reporter vector, integration at sacA, ampr, cmr |
pAH328-derivative [26] |
ECE261 |
BBa_K823025 |
|
Promoters | ||||
| Pveg |
Very strong constitutive promoter |
[27] |
ECE262 |
BBa_K823003 |
| PliaG |
Constitutive promoter |
[28] |
ECE263 |
BBa_K823000 |
| PlepA |
Strong constitutive promoter |
[29] |
ECE264 |
BBa_K823002 |
| J23101 |
Very weak constitutive promoter |
BBa_J23101 |
ECE266 |
BBa_K823005 |
| PliaI |
Bacitracin-inducible promoter |
[30,31] |
ECE267 |
BBa_K823001 |
| PxylA |
Xylose-inducible promoter |
[32] |
ECE268 |
BBa_K823015 |
|
Epitope-tags | ||||
| His10 |
10xHis-tag |
[33] |
ECE269 |
BBa_K823037 |
| FLAG |
FLAG-tag |
[34] |
ECE270 |
BBa_K823034 |
| StrepII |
Streptactin-tag |
[35,36] |
ECE271 |
BBa_K823038 |
| HA |
HA-tag |
[34,37] |
ECE272 |
BBa_K823035 |
| cMyc | cMyc-tag | [34,38] | ECE273 | BBa_K823036 |
aampr, ampicillin resistance; cmr, chloramphenicol resistance; kanr, kanamycin resistance; spcr, spectinomycin resistance; mlsr, erythromycin-induced resistance to macrolide, lincosamide and streptogramin B-antibiotics (MLS).