Table 2.

Vector sources for the Bacillus BioBrick Box

Vector	Description a	Used for	Ref.
pAC6	Vector for transcriptional promoter fusions to lacZ; integrates at amyE; cmr, ampr	pBS1ClacZ	[25]
pAH328	Vector for transcriptional promoter fusions to luxABCDE (luciferase); integrates at sacA; cmr, ampr	pBS3Clux	[26]
pDG1662	Empty vector, integrates at amyE, cmr, spcr, ampr	pBS1C	[23]
pDG1731	Empty vector; integrates at thrC, spcr, mlsr, ampr	pBS4S	[23]
pAX01	Vector for xylose-dependent gene expression; integrates at lacA, mlsr, ampr	pBS2E	[24]
pXT	Vector for xylose-inducible gene expression; integrates in thrC; spcr, ampr	PxylA	[39]
pSB1C3	Replicative E. coli vector, MCS features rfp-cassette; cmr	MCS	[40]
pGFPamy	Vector for transcriptional promoter fusions to gfpmut1; integrates at amyE; cmr, ampr	gfpmut1	[41]

^acm^r, chloramphenicol resistance; kan^r, kanamycin resistance; spc^r, spectinomycin resistance; mls^r, erythromycin-induced resistance to macrolid-, lincosamid- and streptogramin B-antibiotics (MLS); MCS, multiple cloning site.