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. Author manuscript; available in PMC: 2015 Apr 1.
Published in final edited form as: Nat Genet. 2014 Aug 31;46(10):1147–1151. doi: 10.1038/ng.3080

Figure 1. Scc2/Scc4 associates with promoters characterized by broad nucleosome-free regions.

Figure 1

a, ChIP-sequencing analysis of Scc2 and Scc4, three representative regions on chromosome 2 are shown. b, Correlation of Scc2/Scc4 binding sites with transcription factor binding. The total number of bound promoters16 and those with an Scc2/Scc4 peak within 1 kb of the TSS are listed, together with the false discovery rate (FDR, see Methods for details). c, Sequence motifs found at Scc2/Scc4 binding sites. The number of occurrences and the average distance from the Scc2/Scc4 peak summit ± its variance are shown. d, The cohesin loader binds broad nucleosome-free regions. Shown is the averaged nucleosome profile at Scc2/Scc4-bound genes, centered at their TSS (black). The nucleosome profiles of four gene clusters21 in our experimental strain is shown, together with the relative enrichment of Scc2/Scc4-bound genes in these clusters.