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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Mech Dev. 2014 Jul 9;0:36–53. doi: 10.1016/j.mod.2014.06.003

Figure 3. Generation and validation of three deletion lines Df(2R)J15, Df(2R)J16, and Df(2R)J18 in the dKdm2 locus.

Figure 3

(A) Schematic representation of dKdm2 locus and its neighboring genes including Mst85C, beag and Ada. The lower part of the figure shows the three new deletions (bars in grey) generated using four piggyBac insertion lines (blue) and the primers (green arrow) used for their validation. These deletions were validated by the two-sided PCR (B). Note that the extension time for PCR reaction was set so that only short templates (less than 5.0kb, when deletions occur in the deficiency lines) can be amplified. These deficiency lines were further verified using the hybrid PCR (C) and the results are shown in (D). In this assay, PCR products can be detected only when residual piggyBac transposons are present in the expected orientation.