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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Mech Dev. 2014 Jul 9;0:36–53. doi: 10.1016/j.mod.2014.06.003

Figure 7. Effects of loss of dKdm2 on histone lysine modifications as assayed by Western blots.

Figure 7

(A) The levels of histone modifications in the Df(3R)J16 and Df(3R)J18 homozygous mutants during the third instar wandering stage, and w1118 animals at the same stage were used as the control. Similar Western blot analysis was performed to analyze histone modification in the dKdm2d00170 and dKdm2DG12810 homozygous mutants (B), as well as the dKdm2DG12810/Df(3R)J16 and dKdm2DG12810/Df(3R)J18 trans heterozygous larvae (C). (D) dKDM2 protein is depleted in S2-DRSC cells using two different dKdm2 shRNAs (ds-dKdm2-1 and ds-dKdm2-2), and the effects on histone modifications were analyzed by Western blot (E). The dsRNA to white gene was used as the control. These analyses were repeated multiple times.