Table 1.
Evaluation of C3b interaction profiles of compstatin conjugates.[a]
| Peptide | ka [106 m−1 s−1] | kd [10−3 s−1] | KD [nm] |
|---|---|---|---|
| Cp20 | 2.3±0.7 | 6.7±2.9 | 2.9±0.4 |
| ABM1-Cp20 | 1.7±0.1 | 3.5±0.1 | 2.0±0.1 |
| ABM2-Cp20 | 2.7±0.9 | 0.4±0.1 | 0.15±0.06 |
| Cp40 | 2.8±0.6 | 1.3±0.2 | 0.48±0.09 |
| ABM2-Cp40 | 1.8±0.2 | 0.5±0.2 | 0.26±0.09 |
a
Single-cycle kinetic analysis of compstatin conjugates was performed. Sets of five increasing concentrations were consecutively injected over a C3b surface (3000–5000 RU density) in a single cycle. The processed signals were fitted to a 1:1 binding model.