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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: J Neurochem. 2014 Jul 10;131(1):21–32. doi: 10.1111/jnc.12780

Fig. 5.

Fig. 5

Involvement of serpinb1a in PACAP- and NGF-induced survival of PC12 cells. Cells were transfected with control siRNA or siRNA directed against serpinb1a mRNA and were serum-deprived 2 days later. After 12 h in serum-free conditions, cells were treated with PACAP (10−7 M) or NGF (100 ng/ml). (A) Quantification of caspases-3/7 activity after 6 h of treatment with PACAP or NGF. (B) Typical microphotographs illustrating the effect of anti-serpinb1a siRNA on cell survival. Two days after PACAP or NGF treatment, living cells were labeled with calcein (green fluorescence) and dead cells were labeled with ethidium homodimer-1 (red fluorescence). (C) Quantification of cell survival by counting the number of cells after 48 h of treatment with PACAP or NGF in serum-deprived conditions. * p<0.05 versus control in the presence of control siRNA; # p<0.05 versus PACAP in the presence of control siRNA; § p<0.05 versus NGF in the presence of control siRNA. Scale bar = 100 µm.