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. 2014 Sep 19;5:31. doi: 10.1186/2041-9139-5-31

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Copyright © 2014 Lewis and Hong; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Molting-deficient phenotype of Ppa-mlt mutants. Post-hatching stage 2 juvenile (J2) to J3 molt (A)Ppa-mlt(csu26), and (B)Ppa-mlt(csu28). Pre-hatching molt (C)Ppa-mlt(csu26), and (D)Ppa-mlt(csu28). White arrows indicate unshed cuticle from previous stage. The unshed cuticle in the mutants can be observed in the posterior (A, B) and mid-body (C, D) regions - a phenotype not observed in wild-type. Scale bars = 20 μm.