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. 2014 Sep 19;5:31. doi: 10.1186/2041-9139-5-31

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Copyright © 2014 Lewis and Hong; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Expression of the nuclear receptor ultraspiracle (Usp) homolog Ppa-pnhr-1 is expressed prior to the first molt inside the egg. Expression is low in early stage 1 juvenile (J1) (16 hours) and high in late J1 (21 hours), indicative of a pending molt. The J2 developmental arrest serves as the negative control. Data represent eight total qPCR reactions from two independent cDNA samples at each time point. Each cDNA sample was collected from RNA from four pooled synchronized populations. The relative expression ratio of Ppa-pnhr-1 to Ppa-beta-tubulin was normalized to the no-reverse transcriptase negative control. Error bars represent standard error of the mean. **P ≤0.01 (Dunn’s test to J2 arrest).