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. 2014 Sep 24;9(9):e108577. doi: 10.1371/journal.pone.0108577

Figure 1. Supplementation with non-absorbable antibiotics or curcumin improves Western diet induced intestinal barrier dysfunction.

Figure 1

Ten week old LDLR−/− mice were fed ad libitum High Fat High Cholesterol containing Western type diet (TD 88137, WD) for 16 weeks. Experimental groups either received non-absorbable antibiotics (100 mg/L Neomycin and 10 mg/L Polymyxin B) in drinking water or were gavaged daily with Curcumin (100 mg/kg in 0.5% carboxymethylcellulose). Panel A: Fasting plasma was collected at the time of necropsy and circulating LPS levels (EU/ml) were determined. Data are presented as Mean ± SEM, n = 6–9 per group. Panel B: Overnight fasted mice were gavaged with FITC-dextran 4 kDa and plasma samples were collected after 4 h. Appearance of FITC-dextran in plasma was monitored and the data (Mean±SEM, n = 6–9, per group) are presented as FITC concentration (ng/µl). Panel C: Intestinal Alkaline Phosphatase activity was determined and Specific activity (nmoles PNP released/h/mg protein) expressed as % chow-fed controls is shown (Mean ± SEM, n = 6–9 per group). P<0.0001 by One way ANOVA with Bonferroni post-hoc correction; Dissimilar letters above the bars indicate significant differences between groups (P<0.05).