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. 2014 Sep 24;9(9):e108577. doi: 10.1371/journal.pone.0108577

Figure 2. Increased macrophage activation by low dose LPS.

Figure 2

Panel A: Mouse peritoneal macrophages were transduced with AdNF-κB-Luc and treated with LPS (50 pg/ml) or Oleic acid (50 µM) as indicated. Luciferase activity was normalized to cellular protein and data are presented as percent untreated Controls (Mean ± SEM, n = 6). Panel B and C: Conditioned medium was collected after incubation of mouse peritoneal macrophages with LPS with or without oleic acid as indicated and IL-6 and MCP-1 levels were determined by ELISA. Data are expressed as percent of no addition control (Mean ± SEM, n = 6). P<0.0001 by One way ANOVA with Bonferroni post-hoc correction; Dissimilar letters above the bars indicate significant differences between groups (P<0.05).