Table 2. PCR conditions and primers used for the amplification of the ribosomal loci.

Region	Forward	Reverse	Concentration	PCR Protocol
18S-SSU	NS-1: 5′-GTAGTCATATGCTTGTCTC-3′	NS-2: 5′-GGCTGCTGGCACCAGACTTGC-3′	50 pmol/each	94°C for 2 min; 36 cycles of 94°C for 1 min; 57°C for 1 min; 72°C for 2 min; 72°C for 15 min; and 4°C on hold
28S-LSU	NL-1: 5′-GCATATCAATAAGCGGAGGAAAAG-3′	NL-4: 5′-GGTCCGTGTTTCAAGACGG-3′	70 pmol/each	94°C for 2 min; 35 cycles of 94°C for 1 min, 57°C for 1 min; 72°C for 2 min; 72°C for 15 min; and 4°C on hold
ITS	ITS-1: 5′-GTCGTAACAAGGTTAACCTGCGG-3′	ITS-4: 5′-TCCTCCGCTTATTGATATGC-3′	70 pmol/each	94°C for 3 min; 29 cycles of 94°C for 30 s, 57°C for 30 s; 72°C for 45 s; 72°C for 10 min and 4°C on hold