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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Mech Dev. 2014 Apr 19;0:163–176. doi: 10.1016/j.mod.2014.04.001

Figure 1. EGFL7 is expressed by maternal and fetal endothelial cells in the mouse placenta.

Figure 1

In situ hybridization was performed to determine Egfl7 mRNA localization, using an Egfl7 riboprobe on 100μm thick vibratome sections of E10.5 C57Bl/6 placentas (A–E). Higher magnification images of boxes in (A) demonstrating Egfl7 transcript is highly expressed in the maternal decidua (B) and fetal labyrinth (C). Egfl7 sense controls (A inset) show specificity of Egfl7 riboprobe. Paraffin sections of the vibratome sections showing cellular morphology after Egfl7 riboprobe staining in the maternal decidua (D) and fetal labyrinth (E). To determine the cell types expressing EGFL7 protein in the placenta, double immunofluorescent staining was performed on E12.5 C57BL/6 placentas for EGFL7 (red), CD31 (green) and nuclear DAPI (blue) (F–M). EGFL7 colocalizes with the endothelial cell marker, CD31, in the maternal decidua (F–I) and the fetal labyrinth (J–M). Dec-Maternal Decidua, JZ-Junctional Zone, Lab-Fetal Labyrinth. Scale bar (F–M) = 20μm.