Double immunofluorescent staining of normal and preeclamptic (PE) human placentas (A–F) for EGFL7 protein (red), CD31 (green), and nuclear Hoechst (blue) revealing a decrease in EGFL7 protein in PE placentas (arrows-fetal vessels, arrowheads-trophoblasts). Real Time RT-PCR data for EGFL7 (G), CD31 (H), and VEGF (I) on normal and preeclamptic (PE) human placentas demonstrating a significant decrease in EGFL7 transcript levels in PE placentas, even further than the known angiogenic factor, VEGF (n=10, *P<0.05). Scale bars = 50μm.