Figure 1.

2DE phosphoproteome of PBMCs. Representative 2D gel of PBMCs phosphoproteome obtained by isolation of phosphoproteins from total cell lysate using LaCl₃ (A) or affinity micro-columns (B). MW markers:PeppermintStick™ Phosphoprotein MW Standards (Invitrogen™). The arrows indicate four protein spots more expressed in the 2D gel of PBMCs phosphoproteome obtained by isolation of phosphoproteins by LaCl₃, identified by MALDI-TOF-MS/MS as filamin-A (1), coronin-1A (2), pyruvate kinase isoenzyme M1/M2 (3), and ficolin-1 (4) (Table 1).