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Hepatocyte isolation with a modified four-step retrograde perfusion technique. Perfusion was conducted by inserting a suitable pipette into vessels exposed on a cut surface of the sample (A). After sufficient digestion, the liver capsule was mechanically disrupted (B). The emerging cell suspension was filtrated through a 250 μm nylon mesh (C) and centrifuged (50 g, 2 min, 4 °C) (D).
