Figure 3.

Multiple cystoblasts divide and differentiate within a single egg chamber in armi-CycJ mutants. Ovaries from wild type (w¹¹¹⁸) (A, B) and Df(3L)armi-J (C,D) adults stained with anti-hts-RC antibody to visualize ring canals. In wild-type ovarioles each egg chamber contains a single posteriorly located oocyte (arrowheads) with four associated ring canals. (B) shows the boxed region in A at higher magnification. The Df(3L)armi-J mutant ovarioles show multiple abnormalities (C and D). Germarial patterning is disrupted and each egg chamber carries a giant cyst with multiple clusters of 16 cells interconnected by ring canals. (E-J) Ovarioles from wild-type (w¹¹¹⁸) (E, H), Df(3L)armi-J/Df(3L)Exel6095 (F, I), and Df(3L)armi-J (G, J) stained with DAPI (H-J) and Orb (E-G) to visualize oocytes. In wild-type ovarioles each egg chamber contains one Orb-staining oocyte. In the mutant, each giant cyst contains multiple Orb-staining nuclei adjacent to endoreplicating nurse cells. Anterior is towards the top in A and upper left in the rest of the images. Size bars are 50 µm in A-E and H, and 20 µm in F, G, I, and J.