Table 1.
Oligonucleotide primer sequences for quantitative PCR assessment of Guinea pig myosin heavy chain mRNA
| Gene name | Forward primer (5'–3') | Reverse primer (5'–3') | Amplicon size (bp) |
|---|---|---|---|
| MyH1 (MHC IIx) |
TTCATCCAAATGCAGGAAAG |
TCTTTATCTCAAAAGTCATAAATACAA |
90 |
| MyH2 (MHC IIa) |
TGTGGAATGACCAGAGCAAG |
CCTTTGCAATAGGGTAGGACA |
85 |
| MyH4 (MHC IIb) |
TCCATCTACTGCTGCAACG |
ACTCTGCAGATTTTATTTCCTTG |
93 |
| MyH7 (MHC I) | AAGTATCGCAAGGCTCAA | CCTTTCCTTAATTCCAAGC | 129 |
See Tonge et al. for a comprehensive assessment of primer specificity [36].