Table 1.
Time course of carbon partitioning into the major carbon compound classes in the labelled leaf
|
% of
14
C recovered in each fraction related to the total amount per tissue |
|||||||
|---|---|---|---|---|---|---|---|
| After pulse | 15 min chase | 30 min chase | 60 min chase | 180 min chase | |||
|
Labeled leaf
1
|
100 |
100 |
100 |
100 |
100 |
||
| |
Soluble |
77.0 ± 2.2 |
58.4 ± 3.5 |
59.3 ± 2.1 |
46.2 ± 3.4 |
44.7 ± 2.4 |
|
| |
|
Neutral |
14.6 ± 0.9 |
23.1 ± 1.2 |
22.8 ± 1.9 |
11.6 ± 2.0 |
7.3 ± 0.9 |
| |
Acidic |
27.7 ± 0.9 |
15.1 ± 0.7 |
17.0 ± 1.8 |
20.0 ± 1.5 |
25.9 ± 1.8 |
|
| |
Basic |
24.9 ± 1.4 |
14.3 ± 1.2 |
11.3 ± 1.3 |
8.2 ± 0.5 |
5.4 ± 0.5 |
|
| |
Insoluble |
17.3 ± 2.0 |
30.5 ± 4.3 |
36.6 ± 1.9 |
42.5 ± 4.6 |
45.1 ± 4.7 |
|
| |
|
Starch |
16.6 ± 1.1 |
27.7 ± 2.4 |
29.3 ± 2.2 |
34.4 ± 3.1 |
37.2 ± 3.2 |
| |
Protein |
0.8 ± 0.2 |
3.1 ± 0.7 |
3.1 ± 0.5 |
4.6 ± 0.5 |
4.2 ± 0.5 |
|
| |
Others |
0.3 ± 0.1 |
2.0 ± 0.6 |
2.3 ± 0.3 |
3.8 ± 0.2 |
3.7 ± 0.4 |
|
| EtOH soluble | 1.7 ± 0.1 | 4.0 ± 0.6 | 4.1 ± 0.4 | 5.6 ± 0.2 | 5.7 ± 0.3 | ||
1Leaf 8 of Col-0 plants was labelled for 5 min with 14CO2, 6 h into the light, followed by a chase period of 0, 15, 30, 60 or 180 min. After the chase, the labelled leaf was harvested and the incorporation of 14C was determined. The sum of the label in the labelled leaf is set to 100%. The relative amount of 14C incorporated into the different compound classes (as a percentage of the total label in the leaf) is shown. Mean ± SE (n ≥ 5).