Figure 8. Changes in the perinuclear actin stress fibers and nuclear geometry for different rigidities.

(a–d) The observed decrease in stress fiber density as function of substrate rigidity for substrates with elastic modulus 65 kPa, 23 kPa, 5 kPa and 3 kPa respectively (also see panel in Fig. S11). No stress fibers were observed in cell cultured on substrate with elastic modulus 3 kPa. (e–h) The transverse view (3D projections) of the nucleus under different compressive loading for the cells grown on substrates with different rigidities.