Table 1.

Oligonucleotide probes and hybridization conditions used for CARD-FISH analysis of bacteria attached to LDPE fragments

Probe	Nucleotide sequence ( 5′ – 3′)	Probe target	% FA a	° C b	References
NON338	ACT CCT ACG GGA GGC AGC	Negative control	55	35	[34]
EUB338 I	GCT GCC TCC CGT AGG AGT	Most Bacteria	55	35	[35]
EUB338 II	GCA GCC ACC CGT AGG TGT	Planctomycetales	55	35	[36]
EUB338 III	GCT GCC ACC CGT AGG TGT	Verrumicrobiales	55	35	[36]
ARC94	TGC GCC ACT TAG CTG ACA	Arcobacter	20	46	[37]
PSA184	CCC CTT TGG TCC GTA GAC	Pseudoalteromonas, Colwellia	30	31	[38]

^aPer cent (v/v) formamide (FA) in hybridization buffer, based on [39] (NON338, EUB probes), [37] (ARC94) or [38] (PSA184).

^bHybridization temperature, based on [39] (NON338, EUB probes), [37] (ARC94) or a modification of [40] (PSA184).

The fragments were retrieved from microcosms following 14 days of exposure to sediment from sampling site SP2 (Humber Estuary, UK).