Table 1.

HPLC Determinations of the purities of the eight flavonoid phytochemicals

Flavonoids	N a	Wavelength (nm) b	Retention time (min)	Purity (area) (%) c	Purity (height ) (%) d
Quercetin	3	254	6.75 ± 0.01	98.22 ± 0.02	98.49 ± 0.02
Isoquercitrin	3	254	4.51 ± 0.01	97.12 ± 0.02	97.20 ± 0.02
Rutin	3	254	4.40 ± 0.01	99.33 ± 0.03	99.33 ± 0.02
Naringenin	3	254	7.38 ± 0.02	95.64 ± 0.02	95.10 ± 0.02
Naringin	3	254	4.26 ± 0.01	95.06 ± 0.04	95.31 ± 0.03
Hesperetin	3	283	7.83 ± 0.02	95.85 ± 0.03	95.02 ± 0.03
Hesperidin	3	283	4.35 ± 0.02	96.53 ± 0.02	96.78 ± 0.03
Icariin	3	270	9.78 ± 0.02	96.85 ± 0.03	96.29 ± 0.03

^a N = the number of measurements per experiment;

^b The wavelength used for monitoring the elution of flavonoid phytochemicals by HPLC;

^c The purities of eight flavonoid phytochemicals were calculated based on their peak areas;

^d The purities of eight flavonoid phytochemicals were calculated based on their peak heights.