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. 2014 Sep 25;10(9):e1004586. doi: 10.1371/journal.pgen.1004586

Figure 2. Function of the Asp1 phosphatase-like domain.

Figure 2

(A) Diagrammatic representation of the Asp1 protein with the conserved phosphatase signature motif (motif: RH(GNA)XR-HD in Asp1 RHADR-HI)(top) and the Asp1 variants used. Top: (B) Generation of IP7 by GST-Asp1 with varying amounts (2,4,8 µg) of Asp1365-920. Enzymatic reaction was carried out as for 1C. −, component not present; +, component present. (C) Serial dilution patch tests (104–101 cells) of the asp1Δ (deletion) strain expressing the indicated asp1 variants via the nmt1+ promoter. This promoter is repressed in the presence of thiamine and de-repressed in its absence. Cells were grown for 6 days at 25°C on plasmid selective minimal medium without (−) or with (+) TBZ. (D) Serial dilution patch tests (105–101 cells) of the asp1Δ strain expressing either asp11-364, asp1365-920 or asp11-364 and asp1365-920. Cells were grown for 7 days at 25°C on plasmid selective minimal medium without (−) or with (+) TBZ.