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. 2014 Sep 25;10(9):e1004586. doi: 10.1371/journal.pgen.1004586

Figure 4. Asp1 MT regulation functions independently of Mal3.

Figure 4

(A) Serial dilution patch test (104–101 cells) of the indicated strains grown for 5 days at 25°C on YE5S without (−) or with (+) TBZ. (B) Serial dilution patch test (104–101 cells) of the indicated strains grown for 5 days at 25°C on YE5S without (−) or with (+) MBC. (C) Serial dilution patch test of the mal3Δ transformants grown under plasmid selective conditions at 25°C for 5 or 9 days without (−) or with (+) TBZ, respectively. (D) Serial dilution patch tests (105–101 cells) of the indicated strains grown at 25°C on MM without (−) or with (+) TBZ. (E) Photomicrographs of living wild-type, mal3Δ and mal3Δ asp1H397A cells grown at 30°C expressing nmt81::gfp-atb2 +. Bar, 5 µm. (F) MT relative fluorescent intensity (mal3Δ strain, 1+/−0.28, n = 26; mal3Δ asp1H397A strain, 1.25+/−0.46, n = 16; arbitrary units; *, P<0.05 as determined using Welch-Test). (G) Movement of outmost outbound Mal3-GFP comets (see diagram). Speed of comets (nm/sec): wild-type, 56±30, n = 93; asp1H397A, 56±31.6, n = 69; asp1D333A, 83.7±43,8, n = 75. * p<0.0005 for asp1D333A versus wild-type (Welch-test).