Table 1. Interphase MT dynamics in asp1 variant strains.
| strain | growth (nm/sec) | rate of shrinkage (nm/sec) | growth before catastrophe (sec) | length (µm) | pausing at tips (sec) |
| wild-type | 56.8±26.5 (n = 90) | 154.8±66.5 (n = 72) | 81.2±32.3 (n = 100) | 6.3±1.2 (n = 100) | 47.3±38.0 (n = 100) |
| asp1D333A | 66.7±27.2* ↑ (n = 90) | 145.6±67.5 (n = 45) | 68.3±29.4* ↓ (n = 126) | 6.9±1.1* ↑ (n = 86) | 34.5±29.1* ↓ (n = 75) |
| asp1H397A | 58.2±30.9 (n = 90) | 126.7±50.2* ↓ (n = 92) | 99.0±39.2* ↑ (n = 155) | 7.3±1.4* ↑ (n = 100) | 50.7±35.7 (n = 67) |
Parameters of MT dynamics were measured for the indicated strains expressing nmt81::gfp-atb2+. Cells were grown in non-selective minimal medium. n, number of MTs measured; asterisks denote significance between wild-type and mutant; ↓ or ↑, parameter is decreased or increased compared to wild-type. growth: * p<0.01 for asp1D333A vs. wild-type (t-test). Rate of shrinkage: * p<0.005 for asp1H397A vs. wild-type (Welch-test). Growth before catastrophe: * p<0.005 for asp1D333A vs. wild-type (t-test), * p<0.0005 for asp1H397A vs. wild-type (t-test). Length: * p<0.0005 for asp1D333A vs. wild-type (t-test), * p<0.0005 for asp1H397A vs. wild-type (Welch-test). Pausing at tips: * p<0.01 for asp1D333A vs. wild-type (Welch-test).