Figure 1. Early segregation of V. cholerae TerII.

A. Schematic representation of the two sister chromatids of each of the two V. cholerae chromosomes during septation. B. and D. Relative position of dif1 (in black) and dif2 (in red) along the long axis of the cell as a function of cell length in WT (B) or ΔmatP (D) background. C. and E. Frequency of cells with separated dif1 (in black) and dif2 (in red) sisters as a function of cell length in WT (C) or ΔmatP (E) background. The plain red and black lines show the data for the bins containing at least 30 cells (see Figure S1); the dashed grey lines show the data for bins containing 3 to 29 cells (see Figure S1). F. Upper panel, relative number of cells with a single dif1 and dif2 spot (in purple), a single dif1 spot and two dif2 spots or a single dif2 spot and two dif1 spots (in blue) or two dif1 spots and two dif2 spots (in yellow). Cells without either of these numbers of spots were plotted in the ‘other’ category (in red). Middle panel, in the category of cells with 3 spots, relative number of cells with the depicted dispositions of spots in WT or ΔmatP background. Lower panel, in the category of cells with 4 spots, relative number of cells with the depicted dispositions of spots in WT or ΔmatP background. G. Interfocal distance of the sister copies of the dif locus of each of the two V. cholerae chromosomes, (dif1 in black and dif2 in red), as a function of cell length, in WT or ΔmatP background.