Table 1. Neurospora SWI/SNF subunits and knockouts.
Yeast subunits | Function | P value | Neurospora ortholog | Identity of gene deletion mutants in the whole genome knockout collection | Effect of gene deletion on FRQ expression |
SWP59 (ARP9) | Actin related | 1.6e-16 | NCU08840 | FGSC #18403 | Reduced |
FGSC #18404 | |||||
SWP73 (SNF12) | 5.7e-14 | NCU03572 | FGSC #18763 | ND | |
SWI2 (SNF2) | Core subunit, ATPase activity | 4.8e-267 | NCU06488 | FGSC #11467 | ND |
heterokaryon | |||||
SNF5 | Core subunit | 7.4e-40 | NCU00421 | FGSC #11785 | Reduced |
FGSC #11786 | |||||
SWI1 | 4.9e-21 | NCU05891 | FGSC #11904 | Reduced | |
FGSC #11905 | |||||
SWI3 | Core subunit | 7.6e-59 | NCU08003 | FGSC #21999 | ND |
heterokaryon | |||||
SWP82 | 2.3e-10 | NCU03064 | FGSC #19756 | ND | |
SWP29 (TAF14/TAF30) | 2.3e-46 | NCU00444 | FGSC #18696 | ND | |
FGSC #18697 |
Neurospora homologs were recovered based on similarity to Saccharomyces SWI/SNF subunits. All listed subunits are not essential in Saccharomyces cerevisiae. P value refers to the pBLAST score of the Neurospora ortholog when the yeast protein sequence was used to probe the Neurospora genome. In Neurospora, deletions of non essential genes are available as homokaryons whereas deletions of essential genes such as swi2 and swi3 are maintained as heterokaryons in which the deletion is mixed within syncytium with nuclei containing a WT copy of the gene.