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. 2014 Sep 25;10(9):e1004665. doi: 10.1371/journal.pgen.1004665

Figure 5. EZL1 is required for imprecise DNA elimination.

Figure 5

A. Fragmentation of germline DNA downstream of the G51 gene is analyzed by Southern blot hybridization of PstI-digested total genomic DNA run on an 0.8% agarose gel after ICL7 (control) or EZL1 KD. ICL7 is a non essential gene that encodes an infraciliary lattice centrin [56]. The subtelomeric tel51G probe [3] is shown as a grey rectangle above the line. The black square represents telomeric repeats of the MAC chromosome. B. Maternal inheritance of macronuclear deletion is analyzed after ICL7 (control) or EZL1 RNAi, using a cell line reproducibly deleting the ND7 gene from the MAC genome at each sexual generation. PCR analysis was performed on the same DNA samples as in (A) with primers (black arrows) located upstream and downstream of the ND7 open reading frame.