Figure 3.

FoxO1 phosphorylation in 6-days denervated atrophic hind-limb muscles and in 6-days denervated hypertrophic hemidiaphragm muscle. Expression of phosphorylated FoxO1 in 6-days denervated (Den) atrophic anterior tibial muscle (A), atrophic pooled gastrocnemius and soleus muscles (B), and in hypertrophic hemidiaphragm muscle (C) compared to innervated (Inn) and sham operated controls. Representative images of Western blots are shown together with densitometric quantifications. One innervated sample from the respective muscle type was loaded onto all gels as a reference. All samples were measured relative to this reference. The data were normalized to give a mean value of 100.0 in innervated muscles. Mean values ± standard error of the mean. *p < 0.05, **p < 0.01, n = 8 muscles per group.