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. 2014 Sep 25;10(9):e1004656. doi: 10.1371/journal.pgen.1004656

Figure 4. Transgenic expression of SPDEF in prostate epithelium decreased prostate carcinogenesis.

Figure 4

A. Schematic drawing shows Dox-inducible expression of SPDEF and TRAMP transgenes in prostate epithelial cells. B. Decreased prostate carcinogenesis in TRAMP/SPDEF OE mice. Experimental TRAMP/SPDEF OE and control TRAMP mice were given Dox at 4 weeks of age and sacrificed at 25 weeks of age. Mean weight of prostate glands (±SD) was calculated from 7–10 mouse prostates per group. A p value<0.01 is shown with asterisk (**). C. TRAMP/SPDEF OE prostates show increased SPDEF mRNA (left panel) and decreased mRNA levels of cell cycle regulatory genes (right panel). D. Decreased number of proliferating cells in TRAMP/SPDEF OE prostates. Prostate sections were stained with PH3 antibody. The number of PH3-positive cells was counted using 5 random fields in each of 3 individual mice per group. Data represent mean ± SD. A p value<0.01 is shown with (**) and p value<0.05 is shown with (*). Magnification: panels D, 200×.