CaMKII-mediated hyperphosphorylation of the DAT Val559N-terminus. (A) Representative immunoblots of WT DAT and DAT Val559-transfected cells for phosphorylated serines at amino acids 7, 12, and 13 (pSer7, pSer12, and pSer13, respectively), and for total DAT. Inset: immunoblot for dopamine D2 receptor (top) and CaMKII (bottom), confirming their presence in the heterologous cell system. (B) Quantification of pSer DAT band intensities, normalized to total DAT. Figure reproduced from Bowton et al., 2010.