Table 1. Number of genic and non-genic BES-SSR markers from singleton BAC clones designed with BatchPrimer3 and AMMD that were monomorphic, polymorphic or eliminated due to amplification problems in the parental genotyping.
| BES-SSRmarker set | Total numberof SSRs | EliminatedSSRs | Monomorphicmarkers | Polymorphicmarkers | Polymorphismrate (%)a |
| gene-based,BatchPrimer3-SSRs | 32 | 4 | 4 | 24 | 85.7 |
| non-gene based,BatchPrimer3-SSRs | 364 | 82 | 103 | 179 | 63.4 |
| Subtotal | 396 | 86 | 107 | 203 | 65.5 |
| gene-based,AMMD-SSRs | 66 | 10 | 26 | 30 | 53.6 |
| non-gene basedAMMD-SSRs | 437 | 124 | 191 | 122 | 39.0 |
| Subtotal | 503 | 134 | 217 | 152 | 41.2 |
| TOTAL | 899 | 220 | 324 | 357 | 52.4 |
a
In the calculation of the polymorphism rate only monomorphic and polymorphic markers were included.