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. 2014 Sep 25;9(9):e107127. doi: 10.1371/journal.pone.0107127

Figure 3. Endothelial precursors are enriched in the CD45+ cell population.

Figure 3

A, Flow cytometric analysis showed that the majority of cells in regular density (RD) culture became larger after their expansion, whereas the cells in high density (HD) culture maintained the small size of their parental cells (P0). B, Cells from high density culture were analyzed further by gating based on different cell sizes. The small cells showed higher expression levels of EPC related markers than those of the large cells. C, In high density culture, the majority of cells positive for KDR, CD34 and DiI were also positive for CD45. D, In vitro angiogenesis assay of CD45+ and CD45− cells sorted from high density culture. E, CD45+, but not CD45−, cells from high density culture differentiated into endothelial cells that were able to uptake DiI-ac-LDL and express vWF and eNOs. Scale bars, 100 µm. F, Percentages of endothelial cell marker-positive cells in CD45+ and CD45− populations after 14 days of induction (n = 3). *p<0.05.