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. 2014 Sep 25;8(9):e3194. doi: 10.1371/journal.pntd.0003194

Figure 5. BMDC and BMM from cathepsin B-deficient mice express higher levels of IL-12 in response to L. major than cells derived from WT and cathepsin L-deficient mice.

Figure 5

(A) IL-12p70 in supernatants from non-treated BMDC(NT), BMDC infected (Inf) with L. major promastigotes at 48 hours p.i., BMDC stimulated with parasite lysate (LmAg), or with heat-killed parasites (HK), for 48 hours. (B) IL-12p40 and (C) IL-10 concentration in supernatants of BMDC at 48 hours p.i., or stimulation with LmAg or HK parasites. (D) IL-12p70 production in supernatants from non-treated BMM (NT), BMM infected (Inf) with L. major promastigotes at 48 hours p.i., and BMM stimulated for 48 hours with LmAg or HK parasites. (E) IL-12p40 and (F) IL-10 concentration in supernatants of BMM at 48 hours p.i., or stimulation with either LmAg or HK parasites. The results are expressed as mean ± SD of 5 independent experiments. For each experimental group (NT, Inf, LmAg and HK), statistical significance was estimated between WT and Ctsb−/− cells, and between WT and Ctsl−/− cells, * p<0.05, **p<0.01, *** p<0.005.