Figure 7. Th1 polarization of OT-II CD4⁺ naïve T cells by BMDC from WT C57BL/6 and Ctsb^−/− mice.

Isolated CD4⁺ CD25⁻ T cells from OT-II mice were co-cultured with BMDC generated from WT C57BL/6 and Ctsb^−/− mice in the presence of LPS as a stimulus and OVA peptide (327–339) or ovalbumin (OVA) as antigens. A) Zebra plots from one representative experiment. B) Average percentages of IFN-γ, IL-4, or IL-10⁺ CD4⁺ T cells from 3 independent experiments ± SD. * p<0.05.