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. 2014 Sep 25;9(9):e107725. doi: 10.1371/journal.pone.0107725

Figure 2. Purified peripheral eosinophils express FcεRI mRNA.

Figure 2

Peripheral eosinophils were purified via immunomagnetic negative selection to >94% purity (A) and RT-PCR was performed (B). Conditions were optimized using a rat basophil line (RBLSX38) expressing all 3 chains of the human FcεRI. The myeloid cell line, KU812, expressed mRNA for all 3 chains of the receptor, while examination of most BP patients and controls revealed only α- and γ-chain mRNA. Additional control lanes included TNP (template, no primer) and PNT (primer, no template). Representative images are shown; all samples are described in Table III.