Skip to main content
. 2014 Sep 25;9(9):e107842. doi: 10.1371/journal.pone.0107842

Figure 5. Effect of capsaicin on the expression of FASN, ACC, and ACLY protein levels in HepG2 cells.

Figure 5

HepG2 cells were incubated with 0.05, 0.2, and 0.5 mM of capsaicin for 24 h (A, B) and 48 h (C, D), and then the cell lysates were subjected to the SDS-PAGE system. Protein expressions were detected by specific antibodies and. β-actin was used as an internal standard to confirm the integrity and equal protein loading. The bar graph (A, C) shows the quantification of band intensity as a ratio of the individual band intensity relative to the β-actin band intensity in the same blot (e.g. FASN/β-actin). The band (B, D) results shown are representative of those obtained from at least three independent experiments. Data are expressed as means ± SD from at least three separate experiments performed in triplicate, n = 5, *p<0.05.