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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Exp Cell Res. 2014 Jul 9;328(1):99–117. doi: 10.1016/j.yexcr.2014.06.022

Figure 4.

Figure 4

PDCD4 regulates migration and invasion of renal cancer cells via Akt kinase and its downstream effector IKKβ. ACHN and 786-O renal carcinoma cells were transfected with plasmid vectors expressing HA-tagged PDCD4 and HA-tagged constitutively active (CA) HA-Myr-Akt (panels A and B) or CA FLAG-IKKβ (panels C and D) as indicated. (A and C) Migration of ACHN and 786-O renal cancer cells was determined using Boyden chamber assay as described in the Materials and Methods [17]. (B and D) Invasion of the renal cancer cells was determined using collagen-coated membrane as described in the Materials and Methods. Quantification of these results and expression of PDCD4, Myr-Akt and CA IKKβ is shown in the Supplementary Figs. S7A, S7B, S7C and S7D, respectively.