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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Exp Cell Res. 2014 Jul 9;328(1):99–117. doi: 10.1016/j.yexcr.2014.06.022

Figure 5.

Figure 5

PDCD4 regulates mTORC1 activity to control migration and invasion of renal cancer cells. (A) Expression of PDCD4 inhibits mTORC1 activity in renal cancer cells. ACHN and 786-O cells were transfected with PDCD4 expression vector. The cell lysates were immunoblotted with phospho-S6 kinase (Thr-389), S6 kinase and actin antibodies. Same lysates were run in parallel to immunoblot with HA antibody. Quantification of these results is shown in Supplementary Fig. S8. (B and C) Expression of CA mTORC1 reverses PDCD4-induced inhibition of migration (panel B) and invasion (panel C) of renal cancer cells. Both ACHN and 786-O renal cancer cells were separately transfected with PDCD4 and CA mTORC1 expression plasmids as indicated. Migration (panel B) and invasion (panel C) of these cells were measured as described in the Materials and Methods. The quantification of the migration and invasion data and expression of PDCD4 and CA mTORC1 shown in panels B and C is shown in Supplementary Figs. S10A and S10B, respectively.