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. Author manuscript; available in PMC: 2015 Oct 15.
Published in final edited form as: Exp Cell Res. 2014 Jul 9;328(1):99–117. doi: 10.1016/j.yexcr.2014.06.022

Figure 8.

Figure 8

Inhibition of miR-21 increases association of rictor with PDCD4 in renal cancer cells. ACHN and 786-O cells were transfected with miR-21 Sponge. The cell lysates were immunoprecipitated with IgG or PDCD4 antibody followed by immunoblotting with rictor and PDCD4 antibodies (panel A). In panel B, reciprocal immunoprecipitation and immunoblotting were performed. The bottom panels show immunoblotting of the indicated proteins in the cell lysates. Quantification of these results and expression of miR-21 Sponge is shown in Supplementary Fig. S15A and S15B.