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. 2014 Sep 25;10(9):e1004414. doi: 10.1371/journal.ppat.1004414

Figure 6. MIF deficiency correlates with reduced anemia, restored hemoglobin/serum iron levels and restored iron homeostasis during T. brucei infection.

Figure 6

(A) Anemia development during infection in C57Bl/6 (WT, black box; Mif −/−, white box) mice. Results are representative of 2–5 independent experiments and expressed as mean of 3–5 individual mice ± SEM. (B) At day 18 p.i., (left panel) hemoglobin levels and (right panel) serum iron levels in Mif −/− (open bars) and WT (black bars) mice. (C) Expression levels of the iron-homeostasis associated genes Hmox1 (iron import), Dmt1 (iron transport), Fth1 (iron storage) and Fpn1 (iron export) were quantified by RT-QPCR in total livers from Mif −/− (white bars) and WT (black bars) mice at day 18 p.i. Gene-expression levels are normalised using s12 and expressed relatively to expression levels of non-infected mice. Results are representative of 2 independent experiments and presented as mean of 3 individual mice ± SEM (*: p-values ≤0.05).