Skip to main content
. 2014 Sep 25;10(9):e1004421. doi: 10.1371/journal.ppat.1004421

Figure 2. Multivalent, clustered MAM7 is necessary to trigger actin rearrangements in host cells.

Figure 2

Attachment of polymer beads coupled to GST-MAM1 (1 mce domain from MAM7, A) or attachment of soluble GST (C) or GST-MAM7 (D) did not cause significant changes in actin phenotype. Attached objects (beads) per cell and % cells with stress fibers were determined from images taken at indicated timepoints (B, E). Data shown are means ± standard deviation from twelve images (4 frames from n = 3). Images shown are of 1 hour time points and are representative of a set of three experiments. Bar, 10 µm. G-actin (blue) and F-actin (red) content of cells treated with MAM (M) or controls (C) was quantified at 1 hour post treatment. Results are means ± s.e.m. from duplicate experiments (F).