Figure 3. The SIF network is accessible to various types of endosomal cargo.

HeLa cells expressing LAMP1-GFP (green) were infected with Salmonella expressing GFP (green, rod-shaped structures). Pulse-chase experiments were performed by addition of 10 nm gold Nanoparticles conjugated with BSA-Rhodamine (red) at various time points prior and after infection. Live cell imaging of infected cells was performed and representative cells are shown. LAMP1-GFP-positive tubules emerged at 3 to 4 h p.i. Note the almost complete co-localization of endosomal cargo with tubular, LAMP1-GFP-positive SIT. Scale bar: 10 µm.