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. 2014 Sep 25;10(9):e1004374. doi: 10.1371/journal.ppat.1004374

Figure 6. RAW264.7 macrophages infected with Salmonella exhibit double membrane SIF.

Figure 6

RAW264.7 cells expressing LAMP1-GFP (green) were seeded in Petri dishes with a gridded coverslip and infected with Salmonella WT expressing mCherry (STM, red). Live cell imaging was performed 12 h p.i. to visualize LAMP1-GFP-positive SIF (A, MIP; C, F, single Z plane). Subsequently, the cells were fixed and processed for CLEM to reveal the ultrastructure. Several low magnification images were stitched to visualize the cell morphology (B). Higher magnification images were used to align LM and TEM images (D, G). Details of LAMP1-GFP-positive double membrane SIF (E, H) are shown. A cell representative of three biological replicates is shown (1–2 technical replicates with each 2–4 cells). Scale bars: 10 µm (A, B), 2 µm (C, D), 500 nm (E).