Table 2. Bacterial strains and plasmids used in this study.
Designation | relevant genotype | Reference, GenBank ID |
S. enterica serotype Typhimurium strains | ||
NCTC12023 | wild type | lab collection |
P2D6 | ssaV::mTn5 | [50] |
P3H3 | sifA::mTn5 | [15] |
HH107 | ΔsseF::aphT | [54] |
HH108 | ΔsseG::aphT | [54] |
Plasmids | ||
pEGFP-C1 | Clontech | |
pmCherry-C1 | Clontech | |
pFPV25.1 | [51] | |
pFPV-mCherry/2 | [10] | |
p3589 | pETcoco1 const. mCherry | [52] |
p3432 | LAMP1-eGFP | [9] |
p3451 | LAMP1-mCherry | this study |
pBABEpuro GFP-LC3 | GFP-LC3b | [55], Addgene 22405, U05784 |
pmCherry-ATG5 | ATG5 | [33], Addgene 13095, AB048349 |
pmCherry-ATG5-K130R | ATG5-K130R | [33], Addgene 13096 |
p3942 | GFP::sifA in pEGFP | this study |
p2643 | PsseA sscB sseF::HA | [36] |
p3349 | PsseA sscB sseF Δ200–205::HA | [36] |